Heat pretreatment increases resolution in DNA flow cytometry of paraffin-embedded tumor tissue
نویسندگان
چکیده
منابع مشابه
DNA flow cytometry and histopathological grading of paraffin-embedded prostate biopsy specimens in a survival study.
Methods to disintegrate old paraffin-embedded tissue blocks for the application of DNA flow cytometry open up new possibilities for retrospective studies on the correlation between tumor cell nuclear DNA pattern and prognosis of the neoplastic disease. In the present work we used such a method to study the relationship between DNA ploidy, histopathological grade, and survival for 50 patients wi...
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Cell suspensions were prepared from fresh and paraffin embedded samples of lymph nodes from nine patients with non-Hodgkin's lymphoma. DNA flow cytometry was performed on these samples and the results from fresh tissue compared with those from paraffin embedded material. Results were compared in terms of DNA index (as a measure of aneuploidy) and S phase fraction (as an indication of proliferat...
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Flow cytometric analyses of bone marrow cellular DNA content were performed in 1 1 5 adult patients with acute leukemia to assess whether and how proliferative activity relates to other disease and host parameters, to antileukemic effect of induction chemotherapy. and to patient prognosis. Eighty previously untreated patients had a smaller S-phase compartment than patients with morphologically ...
متن کاملDetermining methylation status of methylguanine DNA methyl transferase (MGMT) from formalin-fixed, paraffin embedded tumor tissue
O-6-methylguanine-DNA methyltransferase (MGMT) has been associated with resistance to alkylating agent cancer therapy in Glioblastoma (GBM), the most common and aggressive primary brain tumor in adults. Lower expression or silencing of the MGMT protein by promoter methylation has been reported to improve survival in patients with GBM [1]. This protocol describes bisulfite conversion, methylatio...
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Background: Chronic lupoid leishmaniasis (CLL) is a chronicform of cutaneous leishmaniasis that is usually resistant to antileishmania agents and leishmania is not seen in the histologicsections. We used polymerase chain reaction (PCR) to detectleishmania DNA in CLL specimens.Method: This descriptive cross sectional study was done on20 paraffin embedded specimens of CLL cases referred to theder...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1999
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(19990301)35:3<260::aid-cyto9>3.0.co;2-o